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myhc type 2a  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank myhc type 2a
    Myhc Type 2a, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1081 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myhc+type+2a/anti-Myosin+heavy+chain+Type+IIA/pm41276031-134-31-38
    Average 96 stars, based on 1081 article reviews
    myhc type 2a - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Immune-mediated myogenesis and acetylcholine receptor clustering promote a slow disease progression in ALS mouse models
    Article Snippet: Serial transverse GCM sections were stained with lectin (1:100; Invitrogen) for cellular membranes, and nuclei were marked with Hoechst 33342 (1:1000; Invitrogen 33258). .. To determine the fibre type, sections have been incubated with primary antibodies: MyHC type 1 (1:10; DSHB BA-D5), MyHC type 2a (1:17; DSHB SC-71), MyHC type 2b (1:9; DSHB BF-F3), rabbit anti-laminin (1:100; Sigma L9393), and the respective secondary antibodies: anti-MIgG2b Alexa Fluor 564 (1:500; Invitrogen A21144), anti-MIgG1 Alexa Fluor 488 (1:500; Invitrogen A21121), anti-MIgM Alexa Flour 647 (1:500; Invitrogen A21046), and anti-rabbit Alexa Fluor 405 (1:500; Abcam ab175649). .. To determine embryonic muscle fibres, anti-MyH3 (1:10; DSHB F1652) was used.

    Article Title: Immune-mediated myogenesis and acetylcholine receptor clustering promote a slow disease progression in ALS mouse models.
    Article Snippet: Serial transverse GCM sections were stained with lectin (1:100; Invitrogen) for cellular membranes, and nuclei were marked with Hoechst 33342 (1:1000; Invitrogen 33258). .. To determine the fibre type, sections have been incubated with primary antibodies: MyHC type 1 (1:10; DSHB BA-D5), MyHC type 2a (1:17; DSHB SC-71), MyHC type 2b (1:9; DSHB BF-F3), rabbit anti-laminin (1:100; Sigma L9393), and the respective secondary antibodies: anti-MIgG2b Alexa Fluor 564 (1:500; Invitrogen A21144), anti-MIgG1 Alexa Fluor 488 (1:500; Invitrogen A21121), anti-MIgM Alexa Flour 647 (1:500; Invitrogen A21046), and anti-rabbit Alexa Fluor 405 (1:500; Abcam ab175649). .. To determine embryonic muscle fibres, anti-MyH3 (1:10; DSHB F1652) was used.

    Article Title: Skeletal muscle wasting after burn is regulated by a decrease in anabolic signaling in the early flow phase.
    Article Snippet: Following burns a sustained catabolic stress response is activated, resulting in skeletal muscle wasting.. A better understanding of the underlying mechanisms of postburn skeletal muscle wasting is essential for the development of preventive and/or therapeutic strategies.. Six weeks old female rats underwent a sham, 10% or 40% total body surface area scald burn.

    Immunohistochemistry:

    Article Title: Exercise-mediated alteration of hippocampal Dicer mRNA and miRNAs is associated with lower BACE1 gene expression and Aβ 1-42 in female 3xTg-AD mice
    Article Snippet: .. Immunohistochemistry (IHC) analysis on plantaris muscles was performed as previously described by our laboratory, using antibodies against MyHC Type 1 (BA.D5; DSHB), MyHC Type 2a (SC.71, DSHB), and dystrophin (ab15277; Abcam) that are well-established specific antibodies ( Fry et al. 2017 ). .. Images were captured using a Zeiss upright fluorescent microscope (Zeiss AxioImager M1, Germany) and were quantified using MyoVsion automated analysis software ( Wen et al. 2018 ).

    Muscles:

    Article Title: Exercise-mediated alteration of hippocampal Dicer mRNA and miRNAs is associated with lower BACE1 gene expression and Aβ 1-42 in female 3xTg-AD mice
    Article Snippet: .. Immunohistochemistry (IHC) analysis on plantaris muscles was performed as previously described by our laboratory, using antibodies against MyHC Type 1 (BA.D5; DSHB), MyHC Type 2a (SC.71, DSHB), and dystrophin (ab15277; Abcam) that are well-established specific antibodies ( Fry et al. 2017 ). .. Images were captured using a Zeiss upright fluorescent microscope (Zeiss AxioImager M1, Germany) and were quantified using MyoVsion automated analysis software ( Wen et al. 2018 ).

    other:

    Article Title: Exercise-mediated alteration of hippocampal Dicer mRNA and miRNAs is associated with lower BACE1 gene expression and Aβ 1-42 in female 3xTg-AD mice
    Article Snippet: Images were captured using a Zeiss upright fluorescent microscope (Zeiss AxioImager M1, Germany) and were quantified using MyoVsion automated analysis software ( Wen et al. 2018 ).



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    Effects of antigen retrieval procedures on <t>MyHC</t> immunolabeling. Row 1: Representative images of serial sections of fresh frozen quadriceps muscle preparations (conventional method) demonstrating immunoreactivity with antibodies to MyHC 1, <t>MyHC</t> <t>2a,</t> MyHC 2x, and MyHC 2b. Row 2‐6: Sections of FFPE mouse muscle were stained with antibodies to MyHC 1, MyHC 2a, MyHC 2x, and MyHC 2b to evaluate the effectiveness of antigen unmasking using different antigen retrieval methods. Each image contains two muscles: plantaris on the left, soleus on the right, to include all fiber types in a single image. Row 2 shows that all MyHC antigens are masked on FFPE tissue preparations. NOTE : image brightness was enhanced for tissue visualization. Antigen retrieval methods tested on FFPE sections included HIAR using Sodium Citrate Buffer, pH 6.0 (Row 3), HIAR using EDTA, pH 8.0 (Row 4), HIAR using Tris‐EDTA, pH 9.0 (Row 5), and a combination of HIAR using EDTA, pH 8.0, followed by PIAR using Proteinase K digestion (Row 6). Immunostaining with each antibody was performed individually with antibodies against MyHC 1, MyHC 2a, MyHC 2x, and MyHC 2b. Retrieval with Tris‐EDTA, pH 9.0, and EDTA, pH 8.0, enhanced epitope accessibility for MyHC 1, MyHC 2a, and MyHC 2b, while proteinase K combined with HIAR improved staining for MyHC 2x. However, the combined HIAR and PIAR approach was not effective for labeling MyHC 1, MyHC 2a, and MyHC 2b. Overall, high‐pH antigen retrieval methods were most effective for unmasking MyHC 1, MyHC 2a, and MyHC 2b in FFPE muscle sections. Arrows indicate strongly labeled fibers. Asterisks indicate weakly labeled fibers. Scale bars = 200 µm.
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    Image Search Results


    Effects of antigen retrieval procedures on MyHC immunolabeling. Row 1: Representative images of serial sections of fresh frozen quadriceps muscle preparations (conventional method) demonstrating immunoreactivity with antibodies to MyHC 1, MyHC 2a, MyHC 2x, and MyHC 2b. Row 2‐6: Sections of FFPE mouse muscle were stained with antibodies to MyHC 1, MyHC 2a, MyHC 2x, and MyHC 2b to evaluate the effectiveness of antigen unmasking using different antigen retrieval methods. Each image contains two muscles: plantaris on the left, soleus on the right, to include all fiber types in a single image. Row 2 shows that all MyHC antigens are masked on FFPE tissue preparations. NOTE : image brightness was enhanced for tissue visualization. Antigen retrieval methods tested on FFPE sections included HIAR using Sodium Citrate Buffer, pH 6.0 (Row 3), HIAR using EDTA, pH 8.0 (Row 4), HIAR using Tris‐EDTA, pH 9.0 (Row 5), and a combination of HIAR using EDTA, pH 8.0, followed by PIAR using Proteinase K digestion (Row 6). Immunostaining with each antibody was performed individually with antibodies against MyHC 1, MyHC 2a, MyHC 2x, and MyHC 2b. Retrieval with Tris‐EDTA, pH 9.0, and EDTA, pH 8.0, enhanced epitope accessibility for MyHC 1, MyHC 2a, and MyHC 2b, while proteinase K combined with HIAR improved staining for MyHC 2x. However, the combined HIAR and PIAR approach was not effective for labeling MyHC 1, MyHC 2a, and MyHC 2b. Overall, high‐pH antigen retrieval methods were most effective for unmasking MyHC 1, MyHC 2a, and MyHC 2b in FFPE muscle sections. Arrows indicate strongly labeled fibers. Asterisks indicate weakly labeled fibers. Scale bars = 200 µm.

    Journal: Current Protocols

    Article Title: Adaptable Immunofluorescence Protocol for Muscle Fiber Typing in FFPE Human and Mouse Skeletal Muscle and Intact Mouse Hindlimbs

    doi: 10.1002/cpz1.70246

    Figure Lengend Snippet: Effects of antigen retrieval procedures on MyHC immunolabeling. Row 1: Representative images of serial sections of fresh frozen quadriceps muscle preparations (conventional method) demonstrating immunoreactivity with antibodies to MyHC 1, MyHC 2a, MyHC 2x, and MyHC 2b. Row 2‐6: Sections of FFPE mouse muscle were stained with antibodies to MyHC 1, MyHC 2a, MyHC 2x, and MyHC 2b to evaluate the effectiveness of antigen unmasking using different antigen retrieval methods. Each image contains two muscles: plantaris on the left, soleus on the right, to include all fiber types in a single image. Row 2 shows that all MyHC antigens are masked on FFPE tissue preparations. NOTE : image brightness was enhanced for tissue visualization. Antigen retrieval methods tested on FFPE sections included HIAR using Sodium Citrate Buffer, pH 6.0 (Row 3), HIAR using EDTA, pH 8.0 (Row 4), HIAR using Tris‐EDTA, pH 9.0 (Row 5), and a combination of HIAR using EDTA, pH 8.0, followed by PIAR using Proteinase K digestion (Row 6). Immunostaining with each antibody was performed individually with antibodies against MyHC 1, MyHC 2a, MyHC 2x, and MyHC 2b. Retrieval with Tris‐EDTA, pH 9.0, and EDTA, pH 8.0, enhanced epitope accessibility for MyHC 1, MyHC 2a, and MyHC 2b, while proteinase K combined with HIAR improved staining for MyHC 2x. However, the combined HIAR and PIAR approach was not effective for labeling MyHC 1, MyHC 2a, and MyHC 2b. Overall, high‐pH antigen retrieval methods were most effective for unmasking MyHC 1, MyHC 2a, and MyHC 2b in FFPE muscle sections. Arrows indicate strongly labeled fibers. Asterisks indicate weakly labeled fibers. Scale bars = 200 µm.

    Article Snippet: Monoclonal: Clone SC‐71 , Mouse isotype IgG1 , MyHC 2a , 5.58 μg/ml , DSHB , AB_2147165.

    Techniques: Immunolabeling, Staining, Muscles, Immunostaining, Labeling